Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.

ABSTRACT: We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.

SUBMITTER: Morlan JD 

PROVIDER: S-EPMC3416766 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature