Gene pyramiding of peptidase inhibitors enhances plant resistance to the spider mite Tetranychus urticae.

ABSTRACT: The two-spotted spider mite Tetranychus urticae is a damaging pest worldwide with a wide range of host plants and an extreme record of pesticide resistance. Recently, the complete T. urticae genome has been published and showed a proliferation of gene families associated with digestion and detoxification of plant secondary compounds which supports its polyphagous behaviour. To overcome spider mite adaptability a gene pyramiding approach has been developed by co-expressing two barley proteases inhibitors, the cystatin Icy6 and the trypsin inhibitor Itr1 genes in Arabidopsis plants by Agrobacterium-mediated transformation. The presence and expression of both transgenes was studied by conventional and quantitative real time RT-PCR assays and by indirect ELISA assays. The inhibitory activity o

SUBMITTER: Santamaria ME 

PROVIDER: S-EPMC3416837 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature