Quantifying the rhythm of KaiB-C interaction for in vitro cyanobacterial circadian clock.

ABSTRACT: An oscillator consisting of KaiA, KaiB, and KaiC proteins comprises the core of cyanobacterial circadian clock. While one key reaction in this process--KaiC phosphorylation--has been extensively investigated and modeled, other key processes, such as the interactions among Kai proteins, are not understood well. Specifically, different experimental techniques have yielded inconsistent views about Kai A, B, and C interactions. Here, we first propose a mathematical model of cyanobacterial circadian clock that explains the recently observed dynamics of the four phospho-states of KaiC as well as the interactions among the three Kai proteins. Simulations of the model show that the interaction between KaiB and KaiC oscillates with the same period as the phosphorylation of KaiC, but displays a phas

SUBMITTER: Ma L 

PROVIDER: S-EPMC3416856 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature