Nociceptive and pro-inflammatory effects of dimethylallyl pyrophosphate via TRPV4 activation.

ABSTRACT:

Background and purpose

Sensory neuronal and epidermal transient receptor potential ion channels (TRPs) serve an important role as pain sensor molecules. While many natural and synthetic ligands for sensory TRPs have been identified, little is known about the endogenous activator for TRPV4. Recently, we reported that endogenous metabolites produced by the mevalonate pathway regulate the activities of sensory neuronal TRPs. Here, we show that dimethylallyl pyrophosphate (DMAPP), a substance produced by the same pathway is an activator of TRPV4.

Experimental approach

We examined the effects of DMAPP on sensory TRPs using Ca²⁺ imaging and whole-cell electrophysiology experiments with a heterologous expression system (HEK293T cells transfected with individual TRP channels), cultu

SUBMITTER: Bang S 

PROVIDER: S-EPMC3417458 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature