Soluble epoxide hydrolase: sex differences and role in endothelial cell survival.

ABSTRACT:

Objective

Sex differences in cerebral ischemic injury are, in part, attributable to the differences in cerebrovascular perfusion. We determined whether the brain microvascular endothelial cells (ECs) isolated from the female brain are more resistant to ischemic injury compared with male ECs, and whether the difference is attributable to lower expression of soluble epoxide hydrolase and higher levels of vasoprotective epoxyeicosatrienoic acids (EETs). We also determined whether protection by EETs is linked to the inhibition of rho-kinase (ROCK).

Methods and results

EC ischemic damage was measured after oxygen-glucose deprivation (OGD) using propidium iodide (PI) and cleaved caspase-3 labeling. Expression of soluble epoxide hydrolase was determined by quantitative polymerase c

SUBMITTER: Gupta NC 

PROVIDER: S-EPMC3417749 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature