Rana grylio virus (RGV) 50L is associated with viral matrix and exhibited two distribution patterns.

ABSTRACT:

Background

The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50 L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50 L have not been studied yet.

Methodology/principal findings

We cloned and characterized RGV50L, and revealed 50 L functions in virus assembly and gene regulation. 50 L encoded a 499-amino acid structural protein of about 85 kDa in molecular weight and contained a nuclear localization signal (NLS) and a helix- extension-helix motif. Drug inhibition assay demonstrated that 50 L was an immediate-early (IE) gene. Immuno-fluorescence assay revealed that 50 L appeared early and persisted in RGV-infected cells following two distribution patterns. One

SUBMITTER: Lei XY 

PROVIDER: S-EPMC3418244 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature