Granulocyte-colony stimulating factor improves MDX mouse response to peripheral nerve injury.

ABSTRACT:

Background

G-CSF has been shown to increase neuronal survival, which may positively influence the spinal cord microenvironment during the course of muscular dystrophies.

Methodology/principal findings

Male MDX mice that were six weeks of age received a left sciatic nerve transection and were treated with intraperitoneal injections of 200 µg/kg/day of G-CSF 7 days before and 7 days after the transection. The axotomy was performed after the cycles of muscular degeneration/regeneration, consistent with previous descriptions of this model of muscular dystrophy. C57BL/10 mice were used as control subjects. Seven days after the surgery, the animals were sacrificed and their lumbar spinal cords were processed for immunohistochemistry (anti-MHC I, anti-Synaptophysin, anti-GFAP and a

SUBMITTER: Simoes GF 

PROVIDER: S-EPMC3418329 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature