Analysis of Helicobacter pylori cagA promoter elements required for salt-induced upregulation of CagA expression.

ABSTRACT: Helicobacter pylori infection and consumption of a high-salt diet are each associated with an increased risk for the development of gastric cancer. To investigate potential synergism between these factors, we used a global proteomic approach to analyze H. pylori strains cultured in media containing varying salt concentrations. Among the differentially expressed proteins identified, CagA exhibited the greatest increase in expression in response to high salt concentrations. Analysis of 36 H. pylori strains isolated from patients in two regions of Colombia with differing incidences of gastric cancer revealed marked differences among strains in salt-responsive CagA expression. Sequence analysis of the cagA promoter region in these strains revealed a DNA motif (TAATGA) that was present in eithe

SUBMITTER: Loh JT 

PROVIDER: S-EPMC3418733 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature