The human respiratory syncytial virus nonstructural protein 1 regulates type I and type II interferon pathways.

ABSTRACT: Respiratory syncytial viruses encode a nonstructural protein (NS1) that interferes with type I and III interferon and other antiviral responses. Proteomic studies were conducted on human A549 type II alveolar epithelial cells and type I interferon-deficient Vero cells (African green monkey kidney cells) infected with wild-type and NS1-deficient clones of human respiratory syncytial virus to identify other potential pathway and molecular targets of NS1 interference. These analyses included two-dimensional differential gel electrophoresis and quantitative Western blotting. Surprisingly, NS1 was found to suppress the induction of manganese superoxide dismutase (SOD2) expression in A549 cells and to a much lesser degree Vero cells in response to infection. Because SOD2 is not directly inducibl

SUBMITTER: Hastie ML 

PROVIDER: S-EPMC3418853 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature