GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.

ABSTRACT:

Background

Mesenchymal stromal cells (MSC) have gained importance in tissue repair, tissue engineering and in immunosupressive therapy during the last years. Due to the limited availability of MSC in the bone marrow, ex vivo amplification prior to clinical application is requisite to obtain therapeutic applicable cell doses. Translation of preclinical into clinical-grade large-scale MSC expansion necessitates precise definition and standardization of all procedural parameters including cell seeding density, culture medium and cultivation devices. While xenogeneic additives such as fetal calf serum are still widely used for cell culture, its use in the clinical context is associated with many risks, such as prion and viral transmission or adverse immunological reactions against xeno

SUBMITTER: Fekete N 

PROVIDER: S-EPMC3419200 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature