Liposomal clodronate selectively eliminates microglia from primary astrocyte cultures.

ABSTRACT:

Background

There is increasing interest in astrocyte biology because astrocytes have been demonstrated to play prominent roles in physiological and pathological conditions of the central nervous system, including neuroinflammation. To understand astrocyte biology, primary astrocyte cultures are most commonly used because of the direct accessibility of astrocytes in this system. However, this advantage can be hindered by microglial contamination. Although several authors have warned regarding microglial contamination in this system, complete microglial elimination has never been achieved.

Methods

The number and proliferative potential of contaminating microglia in primary astrocyte cultures were quantitatively assessed by immunocytologic and flow cytometric analyses. To exami

SUBMITTER: Kumamaru H 

PROVIDER: S-EPMC3419615 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature