Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis.

ABSTRACT: Contraction of the cortical actin cytoskeleton underlies both rear retraction in directed cell migration and cytokinesis. Here, we show that talin, a central component of focal adhesions, has a major role in these processes. We found that Dictyostelium talin A colocalized with myosin II in the rear of migrating cells and the cleavage furrow. During directed cell migration, talin A-null cells displayed a long thin tail devoid of actin filaments, whereas additional depletion of SibA, a transmembrane adhesion molecule that binds to talin A, reverted this phenotype, suggesting a requirement of the link between actomyosin and SibA by talin A for rear retraction. Disruptions of talin A also resulted in detachment of the actomyosin contractile ring from the cell membrane and concomitant regressio

SUBMITTER: Tsujioka M 

PROVIDER: S-EPMC3420178 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature