The interactome of the human respiratory syncytial virus NS1 protein highlights multiple effects on host cell biology.

ABSTRACT: Viral proteins can have multiple effects on host cell biology. Human respiratory syncytial virus (HRSV) nonstructural protein 1 (NS1) is a good example of this. During the virus life cycle, NS1 can act as an antagonist of host type I and III interferon production and signaling, inhibit apoptosis, suppress dendritic cell maturation, control protein stability, and regulate transcription of host cell mRNAs, among other functions. It is likely that NS1 performs these different roles through interactions with multiple host cell proteins. To investigate this and identify cellular proteins that could interact with NS1, we used quantitative proteomics in combination with green fluorescent protein (GFP)-trap immunoprecipitation and bioinformatic analysis. This analysis identified 221 proteins that

SUBMITTER: Wu W 

PROVIDER: S-EPMC3421645 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature