Dataset Information
Neural crest cells pattern the surface cephalic ectoderm during FEZ formation.
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ABSTRACT: Background
Multiple fibroblast growth factor (Fgf) ligands are expressed in the forebrain and facial ectoderm, and vascular endothelial growth factor (VEGF) is expressed in the facial ectoderm. Both pathways activate the MAP kinase cascade and can be suppressed by SU5402. We placed a bead soaked in SU5402 into the brain after emigration of neural crest cells was complete.Results
Within 24 hr we observed reduced pMEK and pERK staining that persisted for at least 48 hr. This was accompanied by significant apoptosis in the face. By day 15, the upper beaks were truncated. Molecular changes in the FNP were also apparent. Normally, Shh is expressed in the frontonasal ectodermal zone and controls patterned growth of the upper jaw. In treated embryos, Shh expression was reduced. Both the structural and molecular deficits were mitigated after transplantation of FNP-derived mesenchymal cells.Conclusions
Thus, mesenchymal cells actively participate in signaling interactions of the face, and the absence of neural crest cells in neurocristopathies may not be merely structural.
SUBMITTER: Hu D
PROVIDER: S-EPMC3422019 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Publications
Neural crest cells pattern the surface cephalic ectoderm during FEZ formation.
Developmental dynamics : an official publication of the American Association of Anatomists 20120401 4
<h4>Background</h4>Multiple fibroblast growth factor (Fgf) ligands are expressed in the forebrain and facial ectoderm, and vascular endothelial growth factor (VEGF) is expressed in the facial ectoderm. Both pathways activate the MAP kinase cascade and can be suppressed by SU5402. We placed a bead soaked in SU5402 into the brain after emigration of neural crest cells was complete.<h4>Results</h4>Within 24 hr we observed reduced pMEK and pERK staining that persisted for at least 48 hr. This was ac ...[more]
