Phosphoproteome of human glioblastoma initiating cells reveals novel signaling regulators encoded by the transcriptome.

ABSTRACT:

Background

Glioblastoma is one of the most aggressive tumors with poor prognosis. Although various studies have been performed so far, there are not effective treatments for patients with glioblastoma.

Methodology/principal findings

In order to systematically elucidate the aberrant signaling machinery activated in this malignant brain tumor, we investigated phosphoproteome dynamics of glioblastoma initiating cells using high-resolution nanoflow LC-MS/MS system in combination with SILAC technology. Through phosphopeptide enrichment by titanium dioxide beads, a total of 6,073 phosphopeptides from 2,282 phosphorylated proteins were identified based on the two peptide fragmentation methodologies of collision induced dissociation and higher-energy C-trap dissociation. The SILAC-b

SUBMITTER: Kozuka-Hata H 

PROVIDER: S-EPMC3422224 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature