Inner ear gene transfection in neonatal mice using adeno-associated viral vector: a comparison of two approaches.

ABSTRACT: Local gene transfection is a promising technique for the prevention and/or correction of inner ear diseases, particularly those resulting from genetic defects. Adeno-associated virus (AAV) is an ideal viral vector for inner ear gene transfection because of its safety, stability, long-lasting expression, and its high tropism for many different cell types. Recently, a new generation of AAV vectors with a tyrosine mutation (mut-AAV) has demonstrated significant improvement in transfection efficiency. A method for inner ear gene transfection via the intact round window membrane (RWM) has been developed in our laboratory. This method has not been tested in neonatal mice, an important species for the study of inherited hearing loss. Following a preliminary study to optimize the experimental prot

SUBMITTER: Xia L 

PROVIDER: S-EPMC3422324 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature