Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.

ABSTRACT:

Background

NF-Y is a transcription factor that recognizes with high specificity and affinity the widespread CCAAT box promoter element. It is formed by three subunits: NF-YA and the NF-YB/NF-YC- heterodimer containing histone fold domains (HFDs). We previously identified a large NF-Y gene family in Arabidopsis thaliana, composed of 29 members, and characterized their expression patterns in various plant tissues.

Methods

We used yeast Two-hybrids assays (Y2H), pull-down and Electrophoretic Mobility Shift Assay (EMSA) in vitro experiments with recombinant proteins to dissect AtNF-YB/AtNF-YC interactions and DNA-binding with different AtNF-YAs.

Results

Consistent with robust conservation within HFDs, we show that heterodimerization is possible among all histone-like subu

SUBMITTER: Calvenzani V 

PROVIDER: S-EPMC3422339 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature