Mesenchymal stem cells derived from human limbal niche cells.

ABSTRACT:

Purpose

We investigated whether human limbal niche cells generate mesenchymal stem cells.

Methods

Limbal niche cells were isolated from the limbal stroma by collagenase alone or following dispase removal of the limbal epithelium (D/C), and cultured on plastic in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), or coated or three-dimensional Matrigel in embryonic stem cell medium with leukemia inhibitory factor and basic fibroblast growth factor. Expression of cell markers, colony-forming units-fibroblast, tri-lineage differentiation, and ability of supporting limbal epithelial stem/progenitor cells were compared to limbal residual stromal cells.

Results

Stromal cells expressing angiogenesis markers were found perivascularly, subjacent to li

SUBMITTER: Li GG 

PROVIDER: S-EPMC3423309 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature