High external pH enables more efficient secretion of alkaline α-amylase AmyK38 by Bacillus subtilis.

ABSTRACT:

Background

Bacillus subtilis genome-reduced strain MGB874 exhibits enhanced production of exogenous extracellular alkaline cellulase Egl-237 and subtilisin-like alkaline protease M-protease. Here, we investigated the suitability of strain MGB874 for the production of α-amylase, which was anticipated to provoke secretion stress responses involving the CssRS (Control secretion stress Regulator and Sensor) system.

Results

Compared to wild-type strain 168, the production of a novel alkaline α-amylase, AmyK38, was severely decreased in strain MGB874 and higher secretion stress responses were also induced. Genetic analyses revealed that these phenomena were attributable to the decreased pH of growth medium as a result of the lowered expression of rocG, encoding glutamate dehydroge

SUBMITTER: Manabe K 

PROVIDER: S-EPMC3424145 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature