Comparison of STR profiling from low template DNA extracts with and without the consensus profiling method.

ABSTRACT:

Background

The consensus profiling method was introduced to overcome the exaggerated stochastic effects associated with low copy number DNA typing. However, little empirical evidence has been provided which shows that a consensus profile, derived from dividing a sample into separate aliquots and including only alleles seen at least twice, gives the most informative profile, compared to a profile obtained by amplifying the entire low template DNA extract in one reaction. Therefore, this study aimed to investigate the quality of consensus profiles compared to profiles obtained using the whole low template extract for amplification.

Methods

A total of 100 pg and 25 pg DNA samples were amplified with the PowerPlex® ESI 16 Kits using 30 or 34 PCR cycles. A total of 100 pg and 25

SUBMITTER: Grisedale KS 

PROVIDER: S-EPMC3424158 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature