In vitro exposures in diesel exhaust atmospheres: resuspension of PM from filters versus direct deposition of PM from air.

ABSTRACT: One of the most widely used in vitro particulate matter (PM) exposures methods is the collection of PM on filters, followed by resuspension in a liquid medium, with subsequent addition onto a cell culture. To avoid disruption of equilibria between gases and PM, we have developed a direct in vitro sampling and exposure method (DSEM) capable of PM-only exposures. We hypothesize that the separation of phases and post-treatment of filter-collected PM significantly modifies the toxicity of the PM compared to direct deposition, resulting in a distorted view of the potential PM health effects. Controlled test environments were created in a chamber that combined diesel exhaust with an urban-like mixture. The complex mixture was analyzed using both the DSEM and concurrently collected filter samples

SUBMITTER: Lichtveld KM 

PROVIDER: S-EPMC3424394 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature