Rapid incorporation kinetics and improved fidelity of a novel class of 3'-OH unblocked reversible terminators.

ABSTRACT: Recent developments of unique nucleotide probes have expanded our understanding of DNA polymerase function, providing many benefits to techniques involving next-generation sequencing (NGS) technologies. The cyclic reversible termination (CRT) method depends on efficient base-selective incorporation of reversible terminators by DNA polymerases. Most terminators are designed with 3'-O-blocking groups but are incorporated with low efficiency and fidelity. We have developed a novel class of 3'-OH unblocked nucleotides, called Lightning Terminators™, which have a terminating 2-nitrobenzyl moiety attached to hydroxymethylated nucleobases. A key structural feature of this photocleavable group displays a 'molecular tuning' effect with respect to single-base termination and improved nucleotide fide

SUBMITTER: Gardner AF 

PROVIDER: S-EPMC3424534 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature