A developmentally regulated Myb domain protein regulates expression of a subset of stage-specific genes in Entamoeba histolytica.

ABSTRACT: Conversion between a cyst and trophozoite stage is essential to disease transmission and pathogenesis in the parasitic protist Entamoeba histolytica. A transcriptomic analysis of E. histolytica cysts and trophozoites has recently been accomplished, but the molecular basis of the regulation of encystation is not known. We have now identified a developmentally regulated Myb protein (belonging to the SHAQKY family of Myb proteins), which controls expression of a subset of amoebic stage-specific genes. Overexpression of the nuclear localized Myb protein resulted in a transcriptome that overlapped significantly with the expression profile of amoebic cysts. Analysis of promoters from genes regulated by the Myb protein identified a CCCCCC promoter motif to which amoebic nuclear protein(s) bind in

SUBMITTER: Ehrenkaufer GM 

PROVIDER: S-EPMC3424735 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature