Transcriptome analysis of the honey bee fungal pathogen, Ascosphaera apis: implications for host pathogenesis.

ABSTRACT:

Background

We present a comprehensive transcriptome analysis of the fungus Ascosphaera apis, an economically important pathogen of the Western honey bee (Apis mellifera) that causes chalkbrood disease. Our goals were to further annotate the A. apis reference genome and to identify genes that are candidates for being differentially expressed during host infection versus axenic culture.

Results

We compared A. apis transcriptome sequence from mycelia grown on liquid or solid media with that dissected from host-infected tissue. 454 pyrosequencing provided 252 Mb of filtered sequence reads from both culture types that were assembled into 10,087 contigs. Transcript contigs, protein sequences from multiple fungal species, and ab initio gene predictions were included as evidence sou

SUBMITTER: Cornman RS 

PROVIDER: S-EPMC3425160 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature