Prevalence and characterization of diarrheagenic Escherichia coli isolated from adults and children in Mangalore, India.

ABSTRACT:

Background

Diarrheal diseases are a major cause of morbidity and mortality in resource-limited countries. Among the bacterial pathogens, diarrheagenic E. coli (DEC) are most frequently implicated in cases of epidemic and endemic diarrhea worldwide. The objective of this study was to determine the prevalence of DEC in stool specimens from patients with acute diarrhea using polymerase chain reaction (PCR).

Materials and methods

Escherichia coli stool samples were collected from 115 hospitalized children and adults with acute diarrhea in Mangalore, a coastal city, in southern India. PCR amplification of eae, bfp, stx, ehx genes were used for detection of enteropathogenic (EPEC) and shigatoxigenic E. coli (STEC), lt and st genes were used for enterotoxigenic E. coli (ETEC) and a

SUBMITTER: Shetty VA 

PROVIDER: S-EPMC3425260 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature