Strand-specific RNA-seq reveals ordered patterns of sense and antisense transcription in Bacillus anthracis.

ABSTRACT:

Background

Although genome-wide transcriptional analysis has been used for many years to study bacterial gene expression, many aspects of the bacterial transcriptome remain undefined. One example is antisense transcription, which has been observed in a number of bacteria, though the function of antisense transcripts, and their distribution across the bacterial genome, is still unclear.

Methodology/principal findings

Single-stranded RNA-seq results revealed a widespread and non-random pattern of antisense transcription covering more than two thirds of the B. anthracis genome. Our analysis revealed a variety of antisense structural patterns, suggesting multiple mechanisms of antisense transcription. The data revealed several instances of sense and antisense expression changes

SUBMITTER: Passalacqua KD 

PROVIDER: S-EPMC3425587 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature