Comparison of Scheffersomyces stipitis strains CBS 5773 and CBS 6054 with regard to their xylose metabolism: implications for xylose fermentation.

ABSTRACT: The various strains of Scheffersomyces stipitis (Pichia stipitis) differ substantially with respect to their ability to ferment xylose into ethanol. Two P. stipitis strains CBS 5773 and CBS 6054 have been most often used in literature but comparison of their performance in xylose fermentation under identical conditions has not been reported so far. Conversion of xylose (22 g/L) by each of these P. stipitis strain was analyzed under anaerobic and microaerobic conditions. Ethanol yields of ∼0.41 g/g were independent of strain and conditions used. Glycerol and acetate were formed in constant yields of 0.006 g/g and 0.02 g/g, respectively. Xylitol formation decreased from ∼0.08 g/g to ∼0.05 g/g upon switch from anaerobic to microaerobic conditions. Specific activities of enzymes of the two-ste

SUBMITTER: Krahulec S 

PROVIDER: S-EPMC3426399 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature