Reprogramming of H3K27me3 is critical for acquisition of pluripotency from cultured Arabidopsis tissues.

ABSTRACT: In plants, multiple detached tissues are capable of forming a pluripotent cell mass, termed callus, when cultured on media containing appropriate plant hormones. Recent studies demonstrated that callus resembles the root-tip meristem, even if it is derived from aerial organs. This finding improves our understanding of the regeneration process of plant cells; however, the molecular mechanism that guides cells of different tissue types to form a callus still remains elusive. Here, we show that genome-wide reprogramming of histone H3 lysine 27 trimethylation (H3K27me3) is a critical step in the leaf-to-callus transition. The Polycomb Repressive Complex 2 (PRC2) is known to function in establishing H3K27me3. By analyzing callus formation of mutants corresponding to different histone modificati

SUBMITTER: He C 

PROVIDER: S-EPMC3426549 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature