HPLC-assisted automated oligosaccharide synthesis.

ABSTRACT: A standard HPLC was adapted to polymer supported oligosaccharide synthesis. Solution-based reagents are delivered using a software-controlled solvent delivery system. The reaction progress and completion can be monitored in real time using a standard UV detector. All steps of oligosaccharide assembly including loading, glycosylation, deprotection, and cleavage can be performed using this setup.

SUBMITTER: Ganesh NV 

PROVIDER: S-EPMC3426652 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature