Proteomic profiling of ATM kinase proficient and deficient cell lines upon blockage of proteasome activity.

ABSTRACT: Ataxia Telangiectasia Mutated (ATM) protein kinase is a key effector in the modulation of the functionality of some important stress responses, including DNA damage and oxidative stress response, and its deficiency is the hallmark of Ataxia Telangiectasia (A-T), a rare genetic disorder. ATM modulates the activity of hundreds of target proteins, essential for the correct balance between proliferation and cell death. The aim of this study is to evaluate the phenotypic adaptation at the protein level both in basal condition and in presence of proteasome blockage in order to identify the molecules whose level and stability are modulated through ATM expression. We pursued a comparative analysis of ATM deficient and proficient lymphoblastoid cells by label-free shotgun proteomic experiments comp

SUBMITTER: Marzano V 

PROVIDER: S-EPMC3426930 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature