Rational, combinatorial, and genomic approaches for engineering L-tyrosine production in Escherichia coli.

ABSTRACT: Although microbial metabolic engineering has traditionally relied on rational and knowledge-driven techniques, significant improvements in strain performance can be further obtained through the use of combinatorial approaches exploiting phenotypic diversification and screening. Here, we demonstrate the combined use of global transcriptional machinery engineering and a high-throughput L-tyrosine screen towards improving L-tyrosine production in Escherichia coli. This methodology succeeded in generating three strains from two separate mutagenesis libraries (rpoA and rpoD) exhibiting up to a 114% increase in L-tyrosine titer over a rationally engineered parental strain with an already high capacity for production. Subsequent strain characterization through transcriptional analysis and whole g

SUBMITTER: Santos CN 

PROVIDER: S-EPMC3427108 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature