Circular permutation prediction reveals a viable backbone disconnection for split proteins: an approach in identifying a new functional split intein.

ABSTRACT: Split-protein systems have emerged as a powerful tool for detecting biomolecular interactions and reporting biological reactions. However, reliable methods for identifying viable split sites are still unavailable. In this study, we demonstrated the feasibility that valid circular permutation (CP) sites in proteins have the potential to act as split sites and that CP prediction can be used to search for internal permissive sites for creating new split proteins. Using a protein ligase, intein, as a model, CP predictor facilitated the creation of circular permutants in which backbone opening imposes the least detrimental effects on intein folding. We screened a series of predicted intein CPs and identified stable and native-fold CPs. When the valid CP sites were introduced as split sites, the

SUBMITTER: Lee YT 

PROVIDER: S-EPMC3427171 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature