Activated fluid transport regulates bacterial-epithelial interactions and significantly shifts the murine colonic microbiome.

ABSTRACT: Within the intestinal mucosa, epithelial cells serve multiple functions to partition the lumen from the lamina propria. As part of their natural function, intestinal epithelial cells actively transport electrolytes with passive water movement as a mechanism for mucosal hydration. Here, we hypothesized that electrogenic Cl(-) secretion, and associated mucosal hydration, influences bacterial-epithelial interactions and significantly influences the composition of the intestinal microbiota. An initial screen of different epithelial secretagogues identified lubiprostone as the most potent agonist for which to define these principles. In in vitro studies using cultured T84 cells, lubiprostone decreased E. coli translocation in a concentration-dependent manner (p < 0.001) and decreased S. typhimu

SUBMITTER: Keely S 

PROVIDER: S-EPMC3427217 | biostudies-literature | 2012 May-Jun

REPOSITORIES: biostudies-literature