Glucocorticoid-induced tumor necrosis factor receptor family-related protein regulates CD4(+)T cell-mediated colitis in mice.

ABSTRACT:

Background & aims

The glucocorticoid-induced tumor necrosis factor receptor family-related protein (GITR; also called TNFRSF18 or CD357) regulates the T cell-mediated immune response and is present on surfaces of regulatory T (Treg) cells and activated CD4(+) T cells. We investigated the roles of GITR in the development of colitis in mice.

Methods

Chronic enterocolitis was induced by the transfer of wild-type or GITR(-/-) CD4(+) T cells to GITR(-/-) × Rag(-/-) or Rag(-/-) mice. We determined the severity of colitis by using the disease activity index; measured levels of inflammatory cytokines, T cells, and dendritic cells; and performed histologic analysis of colon samples.

Results

Transfer of nonfractionated CD4(+) cells from wild-type or GITR(-/-) donors induced col

SUBMITTER: Liao G 

PROVIDER: S-EPMC3428052 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature