Plasmid DNA is internalized from the apical plasma membrane of the salivary gland epithelium in live animals.

ABSTRACT: Non-viral-mediated gene delivery represents an alternative way to express the gene of interest without inducing immune responses or other adverse effects. Understanding the mechanisms by which plasmid DNAs are delivered to the proper target in vivo is a fundamental issue that needs to be addressed in order to design more effective strategies for gene therapy. As a model system, we have used the submandibular salivary glands in live rats and we have recently shown that reporter transgenes can be expressed in different cell populations of the glandular epithelium, depending on the modality of administration of plasmid DNA. Here, by using a combination of immunofluorescence and intravital microscopy, we have explored the relationship between the pattern of transgenes expression and the intern

SUBMITTER: Sramkova M 

PROVIDER: S-EPMC3428210 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature