Cationic lipid-coated PEI/DNA polyplexes with improved efficiency and reduced cytotoxicity for gene delivery into mesenchymal stem cells.

ABSTRACT:

Background

Effective gene transfection without serum deprivation is a prerequisite for successful stem cell-based gene therapy. Polyethylenimine (PEI) is an efficient nonviral gene vector, but its application has been hindered by serum sensitivity and severe cytotoxicity.

Methods

To solve this problem, a new family of lipopolyplexes was developed by coating PEI/DNA polyplexes with three serum-resistant cationic lipids, namely, lysinylated, histidylated, and arginylated cholesterol. The physical properties, transfection efficiency, cellular uptake, subcellular distribution, and cytotoxicity of the lipopolyplexes was investigated.

Results

The outer coat composed of lysinylated or histidylated cholesterol remarkably improved the transfection efficiency of the polyplex wi

SUBMITTER: Song H 

PROVIDER: S-EPMC3428250 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature