Analysis of ZAP70 expression in adult acute lymphoblastic leukaemia by real time quantitative PCR.

ABSTRACT:

Background

ZAP70 gene expression is associated with poor prognosis in B-cell lymphoproliferative disorders especially chronic lymphocytic leukaemia (CLL) but its role in adult B-ALL has not been established. On diagnostic samples from 76 patients with adult ALL (65 with B-ALL and 11 with T-ALL) ZAP70 mRNA expression levels were studied by real time-quantitative PCR (RT-qPCR) analysis.

Findings

A broad distribution of ZAP70 expression was observed in ALL, ranging from 0.002 to 5.3 fold that of the ZAP70 positive Jurkat reference cell line. No association was observed between expression levels and the presence of specific cytogenetic abnormalities. Five cases, including one case of T-ALL, had ZAP70 expression above the level of the Jurkat reference cell line.

Conclusions

SUBMITTER: Chakupurakal G 

PROVIDER: S-EPMC3428655 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature