Identification of avian W-linked contigs by short-read sequencing.

ABSTRACT:

Background

The female-specific W chromosomes and male-specific Y chromosomes have proven difficult to assemble with whole-genome shotgun methods, creating a demand for new approaches to identify sequence contigs specific to these sex chromosomes. Here, we develop and apply a novel method for identifying sequences that are W-specific.

Results

Using the Illumina Genome Analyzer, we generated sequence reads from a male domestic chicken (ZZ) and mapped them to the existing female (ZW) genome sequence. This method allowed us to identify segments of the female genome that are underrepresented in the male genome and are therefore likely to be female specific. We developed a Bayesian classifier to automate the calling of W-linked contigs and successfully identified more than 60 nove

SUBMITTER: Chen N 

PROVIDER: S-EPMC3428670 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature