Optogenetic silencing strategies differ in their effects on inhibitory synaptic transmission.

ABSTRACT: Optogenetic silencing using light-driven ion fluxes permits rapid and effective inhibition of neural activity. Using rodent hippocampal neurons, we found that silencing activity with a chloride pump can increase the probability of synaptically evoked spiking after photoactivation; this did not occur with a proton pump. This effect can be accounted for by changes to the GABA(A) receptor reversal potential and demonstrates an important difference between silencing strategies.

SUBMITTER: Raimondo JV 

PROVIDER: S-EPMC3428858 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature