Direct and indirect involvement of microRNA-499 in clinical and experimental cardiomyopathy.

ABSTRACT:

Rationale

MicroRNA-499 and other members of the myomiR family regulate myosin isoforms in pressure-overload hypertrophy. miR-499 expression varies in human disease, but results of mouse cardiac miR-499 overexpression are inconsistent, either protecting against ischemic damage or aggravating cardiomyopathy after pressure overload. Likewise, there is disagreement over direct and indirect cardiac mRNAs targeted in vivo by miR-499.

Objective

To define the associations between regulated miR-499 level in clinical and experimental heart disease and modulation of its predicted mRNA targets and to determine the consequences of increased cardiac miR-499 on direct mRNA targeting, indirect mRNA modulation, and on myocardial protein content and posttranslational modification.

Methods

SUBMITTER: Matkovich SJ 

PROVIDER: S-EPMC3429338 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature