Autoantibodies to heat shock protein 60 promote thrombus formation in a murine model of arterial thrombosis.

ABSTRACT:

Background and objectives

Anti-heat shock protein (HSP)60 autoantibodies are associated with atherosclerosis and are known to affect endothelial cells in vitro. However, their role in thrombus formation remains unclear. We hypothesized that anti-HSP60 autoantibodies could potentiate thrombosis, and evaluated the effect of anti-murine HSP60 antibodies in a ferric chloride (FeCl3)-induced murine model of carotid artery injury.

Methods

Anti-HSP60, or control, IgG was administered to BALB/c mice 48 h prior to inducing carotid artery injury, and blood flow was monitored using an ultrasound probe.

Results

Thrombus formation was more rapid and stable in anti-HSP60 IGG-treated mice than in controls (blood flow=1.7%+/-0.6% vs. 34%+/-12.6%, P=0.0157). Occlusion was complete in

SUBMITTER: Dieude M 

PROVIDER: S-EPMC3429411 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature