Transcriptome analysis of Nicotiana tabacum infected by Cucumber mosaic virus during systemic symptom development.

ABSTRACT: Virus infection of plants may induce a variety of disease symptoms. However, little is known about the molecular mechanism of systemic symptom development in infected plants. Here we performed the first next-generation sequencing study to identify gene expression changes associated with disease development in tobacco plants (Nicotiana tabacum cv. Xanthi nc) induced by infection with the M strain of Cucumber mosaic virus (M-CMV). Analysis of the tobacco transcriptome by RNA-Seq identified 95,916 unigenes, 34,408 of which were new transcripts by database searches. Deep sequencing was subsequently used to compare the digital gene expression (DGE) profiles of the healthy plants with the infected plants at six sequential disease development stages, including vein clearing, mosaic, severe chloro

SUBMITTER: Lu J 

PROVIDER: S-EPMC3429483 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature