Comparative transcript profiling of a male sterile cybrid pummelo and its fertile type revealed altered gene expression related to flower development.

ABSTRACT: Male sterile and seedless characters are highly desired for citrus cultivar improvement. In our breeding program, a male sterile cybrid pummelo, which could be considered as a variant of male fertile pummelo, was produced by protoplast fusion. Herein, ecotopic stamen primordia initiation and development were detected in this male sterile cybrid pummelo. Histological studies revealed that the cybrid showed reduced petal development in size and width, and retarded stamen primordia development. Additionally, disorganized cell proliferation was also detected in stamen-like structures (fused to petals and/or carpel). To gain new insight into the underlying mechanism, we compared, by RNA-Seq analysis, the nuclear gene expression profiles of floral buds of the cybrid with that of fertile pummelo.

SUBMITTER: Zheng BB 

PROVIDER: S-EPMC3429507 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature