The diversity of inducible and constitutively expressed erm(C) genes and association to different replicon types in staphylococci plasmids.

ABSTRACT: The aim of this study was to analyze the diversity of the macrolide resistance gene, erm(C) in relation to structural alterations affecting the gene expression. In addition, the association of erm(C) to mobile genetic elements (MGEs) in staphylococci mainly from Danish pigs was investigated. In total, 78 erythromycin-resistant isolates were screened for erm(C) by PCR. The erm(C) genes incl. the upstream regulatory region were sequenced and the expression types were characterized phenotypically (agar diffusion test) and genotypically (sequence analysis). Phylogenetic analysis of erm(C) was compared with structural alterations affecting the gene expression. Plasmids carrying erm(C) from seven selected isolates were fully or partially sequenced. Thirty-seven isolates were shown to be erm(C) p

SUBMITTER: de Vries LE 

PROVIDER: S-EPMC3429524 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature