Sampling strategy and potential utility of indels for DNA barcoding of closely related plant species: a case study in taxus.

ABSTRACT: Although DNA barcoding has become a useful tool for species identification and biodiversity surveys in plant sciences, there remains little consensus concerning appropriate sampling strategies and the treatment of indels. To address these two issues, we sampled 39 populations for nine Taxus species across their entire ranges, with two to three individuals per population randomly sampled. We sequenced one core DNA barcode (matK) and three supplementary regions (trnH-psbA, trnL-trnF and ITS) for all samples to test the effects of sampling design and the utility of indels. Our results suggested that increasing sampling within-population did not change the clustering of individuals, and that meant within-population P-distances were zero for most populations in all regions. Based on the markers

SUBMITTER: Liu J 

PROVIDER: S-EPMC3430262 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature