Parallel genetic changes and nonparallel gene-environment interactions characterize the evolution of drug resistance in yeast.

ABSTRACT: Beneficial mutations are required for adaptation to novel environments, yet the range of mutational pathways that are available to a population has been poorly characterized, particularly in eukaryotes. We assessed the genetic changes of the first mutations acquired during adaptation to a novel environment (exposure to the fungicide, nystatin) in 35 haploid lines of Saccharomyces cerevisiae. Through whole-genome resequencing we found that the genomic scope for adaptation was narrow; all adapted lines acquired a mutation in one of four late-acting genes in the ergosterol biosynthesis pathway, with very few other mutations found. Lines that acquired different ergosterol mutations in the same gene exhibited very similar tolerance to nystatin. All lines were found to have a cost relative to wi

SUBMITTER: Gerstein AC 

PROVIDER: S-EPMC3430539 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature