Improving the performance of true single molecule sequencing for ancient DNA.

ABSTRACT:

Background

Second-generation sequencing technologies have revolutionized our ability to recover genetic information from the past, allowing the characterization of the first complete genomes from past individuals and extinct species. Recently, third generation Helicos sequencing platforms, which perform true Single-Molecule DNA Sequencing (tSMS), have shown great potential for sequencing DNA molecules from Pleistocene fossils. Here, we aim at improving even further the performance of tSMS for ancient DNA by testing two novel tSMS template preparation methods for Pleistocene bone fossils, namely oligonucleotide spiking and treatment with DNA phosphatase.

Results

We found that a significantly larger fraction of the horse genome could be covered following oligonucleotide spikin

SUBMITTER: Ginolhac A 

PROVIDER: S-EPMC3430569 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature