Transcriptome and proteome dynamics of a light-dark synchronized bacterial cell cycle.

ABSTRACT:

Background

Growth of the ocean's most abundant primary producer, the cyanobacterium Prochlorococcus, is tightly synchronized to the natural 24-hour light-dark cycle. We sought to quantify the relationship between transcriptome and proteome dynamics that underlie this obligate photoautotroph's highly choreographed response to the daily oscillation in energy supply.

Methodology/principal findings

Using RNA-sequencing transcriptomics and mass spectrometry-based quantitative proteomics, we measured timecourses of paired mRNA-protein abundances for 312 genes every 2 hours over a light-dark cycle. These temporal expression patterns reveal strong oscillations in transcript abundance that are broadly damped at the protein level, with mRNA levels varying on average 2.3 times more tha

SUBMITTER: Waldbauer JR 

PROVIDER: S-EPMC3430701 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature