Derivation of a novel undifferentiated human foetal phenotype in serum-free cultures with BMP-2.

ABSTRACT: Skeletal stem and progenitor populations provide a platform for cell-based tissue regeneration strategies. Optimized conditions for ex vivo expansion will be critical and use of serum-free culture may allow enhanced modelling of differentiation potential. Maintenance of human foetal femur-derived cells in a chemically defined medium (CDM) with activin A and fibroblast growth factor-2 generated a unique undifferentiated cell population in comparison to basal cultures, with significantly reduced amino acid depletion, appearance and turnover, reduced alkaline phosphatase (ALP) activity and loss of type I and II collagen expression demonstrated by fluorescence immunocytochemistry. Microarray analysis demonstrated up-regulation of CLU, OSR2, POSTN and RABGAP1 and down-regulation of differentiat

SUBMITTER: Mirmalek-Sani SH 

PROVIDER: S-EPMC3430854 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature