Gene expression and proteomic analysis of the formation of Phakopsora pachyrhizi appressoria.

ABSTRACT:

Background

Phakopsora pachyrhizi is an obligate fungal pathogen causing Asian soybean rust (ASR). A dual approach was taken to examine the molecular and biochemical processes occurring during the development of appressoria, specialized infection structures by which P. pachyrhizi invades a host plant. Suppression subtractive hybridization (SSH) was utilized to generate a cDNA library enriched for transcripts expressed during appressoria formation. Two-dimensional gel electrophoresis and mass spectroscopy analysis were used to generate a partial proteome of proteins present during appressoria formation.

Results

Sequence analysis of 1133 expressed sequence tags (ESTs) revealed 238 non-redundant ESTs, of which 53% had putative identities assigned. Twenty-nine of the non-redundan

SUBMITTER: Stone CL 

PROVIDER: S-EPMC3431228 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature